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fast gene expression analysis  (fluidigm)


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    Structured Review

    fluidigm fast gene expression analysis
    Fast Gene Expression Analysis, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 716 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fast+gene+expression+analysis/96%2E96+Dynamic+Array+IFC+for+Gene+Expression/pm41861829-366-11-10
    Average 95 stars, based on 716 article reviews
    fast gene expression analysis - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Expressing:

    Article Title: Integrated drivers of basal and acute immunity in diverse human populations.
    Article Snippet: .. The expression of 96 immune genes was measured using the Fluidigm Fast Gene Expression Analysis Using EvaGreen on the BioMark HD System and 96.96 Dynamic Array integrated fluidic circuits. .. Primers were designed using Fluidigm’s D3 Assay Design Tool.

    Article Title: Osteopontin regulates the cross-talk between phosphatidylcholine and cholesterol metabolism in mouse liver
    Article Snippet: .. The expression of the selected genes was measured by RT-qPCR using the BioMarkTM HD system in combination with Dynamic Array Integrated Fluidic Circuits (IFC) (Fluidigm Corporation) following Fluidigm’s Fast Gene Expression Analysis using EvaGreen on the BioMark HD System version D1 protocol. ..

    Gene Expression:

    Article Title: Integrated drivers of basal and acute immunity in diverse human populations.
    Article Snippet: .. The expression of 96 immune genes was measured using the Fluidigm Fast Gene Expression Analysis Using EvaGreen on the BioMark HD System and 96.96 Dynamic Array integrated fluidic circuits. .. Primers were designed using Fluidigm’s D3 Assay Design Tool.

    Article Title: Osteopontin regulates the cross-talk between phosphatidylcholine and cholesterol metabolism in mouse liver
    Article Snippet: .. The expression of the selected genes was measured by RT-qPCR using the BioMarkTM HD system in combination with Dynamic Array Integrated Fluidic Circuits (IFC) (Fluidigm Corporation) following Fluidigm’s Fast Gene Expression Analysis using EvaGreen on the BioMark HD System version D1 protocol. ..

    Article Title: Comprehensive discovery and analysis of RNA binding protein-dependent post-transcriptional events in mammalian systems /
    Article Snippet: 24 hours later 150ng of the prepped shRNA plasmid was mixed with 0.2ul Polyplus jetPRIME transfection reagent diluted into JetPrime Buffer using the BioMek FXP and incubated at RT for 10 minutes. .. Fast Gene Expression Analysis Using EvaGreen on the BioMark HD System (Fluidigm) was performed according to the Advanced Development Protocol 37 for the 96.96 Dynamic Array IFC. .. Specifically, we conducted specific target amplification (STA) reactions for 14 cycles, Exonuclease I treatment, and diluted the products 10-fold with TE Buffer (TEKnova).

    Article Title: Inflammation triggers ILC3 patrolling of the intestinal barrier.
    Article Snippet: To synthesize complementary DNA, PCR with reverse transcription was performed using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche). .. From the cDNA, we followed the protocol ‘Fast Gene Expression Analysis Using Evagreen on the Biomark’ from Fluidigm. .. Briefly, preamplified cDNA was obtained after a preamplification step with Delta Genes Assays (Fluidigm) using the TaqMan PreAmp Master Mix (Applied Biosystems) and a subsequent purification step using exonuclease I (New England Biolabs) and was then diluted 1:5 in Tris EDTA buffer (Invitrogen).

    Article Title: Bile acid profiles and mRNA expression of bile acid-related genes in the liver of dairy cows with high versus normal body condition.
    Article Snippet: .. Primers were measured in triplicate using the Biomark HD RT-qPCR system and 96.96 integrated fluidic circuits (IFC) prepared according to the protocol “Fast Gene Expression Analysis Using EvaGreen on Biomark HD for Biomark” by Fluidigm. ..

    Article Title: Bile acid profiles and mRNA abundance of bile acid-related genes in adipose tissue of dairy cows with high versus normal body condition.
    Article Snippet: In brief, samples were measured in triplicates using the Biomark HD RT-qPCR system and 96.96 integrated fluidic circuits (IFCs). .. Preparation of the IFCs was performed according to the protocol “Fast Gene Expression Analysis Using EvaGreen on Biomark HD or Biomark” from Fluidigm. ..

    Article Title: Inflammation triggers ILC3 patrolling of the intestinal barrier
    Article Snippet: To synthesize complementary DNA, PCR with reverse transcription was performed using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche). .. From the cDNA, we followed the protocol ‘Fast Gene Expression Analysis Using Evagreen on the Biomark’ from Fluidigm. .. Briefly, preamplified cDNA was obtained after a preamplification step with Delta Genes Assays (Fluidigm) using the TaqMan PreAmp Master Mix (Applied Biosystems) and a subsequent purification step using exonuclease I (New England Biolabs) and was then diluted 1:5 in Tris EDTA buffer (Invitrogen).

    Quantitative RT-PCR:

    Article Title: Osteopontin regulates the cross-talk between phosphatidylcholine and cholesterol metabolism in mouse liver
    Article Snippet: .. The expression of the selected genes was measured by RT-qPCR using the BioMarkTM HD system in combination with Dynamic Array Integrated Fluidic Circuits (IFC) (Fluidigm Corporation) following Fluidigm’s Fast Gene Expression Analysis using EvaGreen on the BioMark HD System version D1 protocol. ..

    Article Title: Bile acid profiles and mRNA expression of bile acid-related genes in the liver of dairy cows with high versus normal body condition.
    Article Snippet: .. Primers were measured in triplicate using the Biomark HD RT-qPCR system and 96.96 integrated fluidic circuits (IFC) prepared according to the protocol “Fast Gene Expression Analysis Using EvaGreen on Biomark HD for Biomark” by Fluidigm. ..



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